过表达miR-193b对黑色素细胞中MITF和TYR表达的影响

doi:10.11843/j.issn.0366-6964.2016.05.010

Abstract

Abstract:

MITF and TYR have been identified to be crucial in the process of melanogenesis，the study aims to investigate the relationship between MITF，TYR and miR-193b，and then to determine the functional roles of miR-193b in the regulation of melanogenesis in melanocyte.The melanocytes were transfected with the miR-193b plasmid，tested the assay of melanin，and analyzed expression patterns of MITF and TYR using real-time PCR and Western blotting.The results indicated that over-expression of miR-193b in melanocyes reduced the assay of melanin and decreased the expression of MITF and TYR both at the mRNA and protein level，the level of MITF mRNA and TYR mRNA were reduced to 0.233 times (P<0.01) and 0.198 times (P<0.01) respectively，the level of MITF and TYR proteins were reduced to 0.232 times and 0.321 times which was significantly different.Results support the role of miR-193b in melanocytes indirectly，which regulated the melanogenesis by MITF and TYR.

CLC Number:

S829.9

ZHANG Ya-ling，XU Dong-mei，DU Bin，XIE Jian-shan，FAN Rui-wen，YU Xiu-ju，ZHU Zhi-wei，DONG Chang-sheng. The Influences of Over-Expressing miR-193b on MITF and TYR in Melanocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 938-943.

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The Influences of Over-Expressing miR-193b on MITF and TYR in Melanocytes

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